Molecular identification of ‘Candidatus Phytoplasma solani’ using SecY and Vmp1 Genes in Tomato Plants from Van province

Tomato (Solanum lycopersicum) is one of the most financially important vegetable crops. It a species belonging to Solanaceae family and cultivated in many countries, including Turkey. The natural presence ‘Candidatus Phytoplasma solani’ (‘Ca. P. solani’) from Stolbur group (16SrXII) tomato plants extensively characterized based on 16S rRNA gene worldwide. displaying abnormality sterility flower, purpling, bushy appearance were observed Van province, DNA extraction leaves was performed, extracted used amplify sequences using universal primer pairs by double PCR assays. After confirming probable agent solani’), nested testing performed an appropriate set amplifying SecY Vmp1 genes same DNAs. Amplified yields then cloned into pGEM T-Easy vector sequenced New Generation System (NGS). Sequenced 1438 bp nucleotides for (MN104838) 905 (MN125054) revealed 97.38% 100% maximum nucleotide similarity with formerly published ‘Ca. belonged (16Sr-XII), respectively. According phylogenetic tree created our solani’, Vmp1, showed highly affinity Serbia France, To best knowledge, this study first report reveal relationships stolbur (16Sr XII-A) plant Turkey non-ribosomal genes.